THE 2-MINUTE RULE FOR JWH 018

Rats were fast anesthetized with isoflurane employing a fall jar which contained a raised floor over a gauze pad saturated with five mL of isoflurane. When completely anesthetized, each rat acquired a surgically implanted IPTT-three hundred transponder (Bio Medic Information Systems, Seaford, DE, United states of america) to facilitate the non-inva